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D. E. F. V. Thin-Layer Chromatography Gas Chromatography High-Performance Liquid Chromatography Capillary Electrophoresis Other Analytical Techniques Summary References I. INTRODUCTION The analysis of the methylxanthines (caffeine, theobromine, and theophylline) is important in the areas of nutrition and clinical chemistry. These three compounds compose the majority of the alkaloids present in coffee, tea, cocoa, cola nuts, and guarana. This chapter on analysis of methylxanthines is divided into three sections: historical methods, current analytical methods for foods, and current methods for biological samples which can include plasma, blood, urine, cell extracts, and other potential samples of biological significance.
41. J. A. Jr, The quantitative determination of caffeine in beverages using capillary electrophoresis analysis, 21,389-91,1993. 42. ThermoSeparation Products Application Note UN92-4 , ThermoSeparation Products; San Jose, CA, 1992 43. , Flow injection analysis for hypoxanthine in meat with dissolved oxygen detector and enzyme reactor, Talanta, 43,2053,1996. 44. , Clincial experience with theophylline: Relationship between dosage, serum concentration and toxicity, JAMA, 235,1983,1976. ©1998 CRC Press LLC 45.
The reaction by-product, diethylamine, is extremely volatile and can be removed easily. It is not within the scope of this document to describe the uses of GC in the analytical laboratory in great detail but there are a number of excellent references available on the topic. ©1998 CRC Press LLC D. High-Performance Liquid Chromatography High-performance liquid chromatography (HPLC) is probably one of the most important instrumental methods in analytical chemistry and continues to grow at a rapid rate.