By V. A. Khripach, V. N. Zhabinskii, A. E. de Groot
Within the monograph, the result of medical investigations accomplished via researchers of the Institute of Bioorganic Chemistry of tne nationwide Academy of Sciences of Belarus (Minsk, Belarus) and the dept of natural Chemistry of the Wageningen Agricultural college (Wageningen, The Netherlands) are awarded. The publication covers buildings, category, isolation, spectroscopic decision, biosynthesis, metabolism, physiological mode of motion, functional functions in agriculture, and so forth. of a brand new classification of plant hormones - brassinosteroids.
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Sample text
UV detection was used first with naphthaleneboronic acid (Gamoh et aL, 1988) as a reagent for derivatization (Fig. 13). The detection limit was about 100 pg per injection. -Q. et al, 1995), and (dansylamino)phenylboronic acid (Gamoh et al, 1989b, 1990a; Motegi et al, 1994), show even better results. The BS derivatives can be detected down to 50-20 pg per injection. Another useful variant of HPLC analysis of BS relies on derivatization with ferroceneboronic acid, which enables electrochemical detection (Gamoh et al, 1990b).
This process may play a role in the inactivation of castasterone in plants. Similar studies on the biosynthesis of brassinolide have been performed in seedlings of Nicotiana tabacum and Oryza sativa (Suzuki et al, 1995a). 60 BRASSINOSTEROIDS: A NEW CLASS OF PLANT HORMONES 1200 1200 600 Relative activity Fig. 20. Relative activity of brassinolide and its biogenetic precursors. Despite the fact that the most detailed and large-scale investigations were carried out on cultured cells of Catharanthus roseus only, a similar pathway to brassinolide may be expected for other plants as well.
Analysis of the vicinal coupling constants using the Karplus relationship allows one to draw some conclusions. 23 = 5-9 Hz. , 1997). All the vicinal coupling SPECTRAL PROPERTIES 43 constants of the side chain were determined and the corresponding dihedral angles were calculated. In addition, quantitative 2D NOE measurements and molecular dynamics simulations were carried out. The obtained results showed that brassinolide was able to maintain a preferential conformation. The conformation of epibrassinolide around the C-22 - C-23 bond was suggested to be more flexible, which could explain its weaker biological activity.