By Kee-Yoeup Paek, Hosakatte Niranjana Murthy, Eun-Joo Hahn (auth.), Praveen K. Saxena, S. Mohan Jain (eds.)
Given the important and far-reaching purposes of medicinal plant metabolites around the globe, the standard and consistency of the goods in addition to the very survival of assorted species are of the maximum value. In Protocols for In Vitro Cultures and Secondary Metabolite research of fragrant and Medicinal Plants, specialist researchers supply designated, step by step protocols for the institution of in vitro cultures of key medicinal vegetation, their mass multiplication in a managed surroundings, and step-wise secondary metabolite research, genetic transformation, large-scale metabolite creation in a bioreactor, and molecular markers. moreover, lots of those protocols will offer a foundation for far wanted efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species on the verge of collapse of extinction in addition to efforts to guard them from the antagonistic impression of speedy climatic alterations. As a quantity within the Methods in Molecular Biology™ sequence, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, without difficulty reproducible laboratory protocols, and tips about troubleshooting and heading off identified pitfalls.
Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite research of fragrant and Medicinal Plants is a perfect source for scientists endeavoring to proceed the study in this fascinating ordinary department of medication.
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39). Acknowledgments This work was supported by the Academy of Finland (project numbers 126488 to KN, 121994 to TS, 118569 to AMP, 123826 to HH and 45591 and 64308 to RJT). Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine 51 References 1. , and von Weissenberg, K. (1996). Somatic embryogenesis of Pinus sylvestris. Scand. J. For. Res. 11, 242–250 2. , and Aronen, T. (1999). Somatic embryogenesis of Scots pine: cold treatment and characteristics of explants affecting induction.
1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. A. H. J. J. ) PCR Protocols. Academic Press, San Diego, CA, USA, 315–322 26. , and Toivanen, P. (1995). Use of the polymerase chain reaction and DNA sequencing for detection of Bartonella quintana in the aortic valve of a patient with culture-negative infective endocarditis. Clin. Infect. Dis. 21, 891–896 27. E. (1995). Tannin analysis. Department of Chemistry, Miami University, Oxford, OH 28. , and Cánovas, M.
Fig. 1. Polyphenolic compounds in Scots pine needles. 1 mg/L. Purity of standards is noted in exact concentrations. Purified proanthocyanidins from pine needles and stems (27). 2. 25% o-phosphoric acid and methanol (see Note 1). 3. 6 × 60 mm, 3 mm-Hypersil ODS column (Agilent Technologies; Germany). 4. 1701 capillary GC-column (Perkin-Elmer Elite). 5. Helium as a carrier gas. 6. A boiling water bath and colorimetric equipment. 7. HPLC-diode array detector. 8. GC-MS equipment. 3. Extraction of Monoterpenes 1.