By Mahendra S. Rao, Mohan C. Vemuri (auth.), David Gordon, Neil J. Scolding (eds.)

In the dramatic and quickly constructing box of neural transplantation for CNS fix, the main robust contributor has been the very important study targeting stem cells. In Neural telephone Transplantation: equipment and Protocols, major specialists within the box learn tried-and-true laboratory suggestions so that it will provide scientists with a company starting place upon which extra developments could be dependent. Written within the hugely winning Methods in Molecular Biology™ sequence structure, the chapters of this quantity contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, easily reproducible protocols, and Notes sections, which study pointers on troubleshooting and fending off recognized pitfalls.

Cutting-edge and straightforward to exploit, Neural cellphone Transplantation: tools and Protocols offers the main thorough and crucial protocols that may enable new generations of neuroscientists to go into and give a contribution to this uniquely inspiring field.

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Fischbach, G. D. & McKhann, G. M. (2001). Cell therapy for Parkinson’s disease. N. Engl. J. Med. 344: 763–765. Freed, C. , Greene, P. , Breeze, R. , Tsai, W. , Culver, S. et al. (2001). Transplantation of embryonic dopamine neurons for severe Parkinson’s disease. N. Engl. J. Med. 344: 710–719. Gill, S. , Patel, N. , Hotton, G. , Brooks, D. , Svendsen, C. N. & Heywood, P. (2003). Direct brain infusion of glial cell line-derived neurotrophic factor in Parkinson disease. Nat. Med. 9: 589–595. Eglitis, M.

Et al. (2006). Transplantation of human embryonic stem cellderived cells to a rat model of Parkinson’s disease: effect of in vitro differentiation on graft survival and teratoma formation. Stem Cells 24: 1433–1440. Dunnett, S. B. & Rosser, A. E. (2007). Stem cell transplantation for Huntington’s disease. Exp. Neurol. 203: 279–292. Rosser, A. E. & Dunnett, S. B. (2003). Neural transplantation in patients with Huntington’s disease. CNS Drugs 17: 853–867. Philpott, L. , Kopyov, O. , Lee, A. , Duma, C.

Neurosci. Res. 80: 611–619. 70. , Liang, L. & Xu, J. (2005). Glial cell line-derived neurotrophic factor in bone marrow stromal cells of rat. Neuroreport 16: 581–584. 71. , de la Cuetara, K. & Pavon, N. (2004). Bone marrow stromal cells produce nerve growth factor and glial cell line-derived neurotrophic factors. Biochem. Biophys. Res. Commun. 316: 753–754. 72. , Ourednik, V. & Ourednik, J. (2005). Grafted neural stem cells shield the host environment from oxidative stress. Ann. N Y Acad. Sci. 1049: 185–188.

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Neural cell transplantation: methods and protocols by Mahendra S. Rao, Mohan C. Vemuri (auth.), David Gordon, Neil
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