By Jaroslav Dolezel, Johann Greilhuber, Jan Suda
Specific at newbies in addition to skilled clients, this convenient reference explains the advantages and makes use of of circulate cytometery within the learn of vegetation and their genomes. Following a short advent that highlights basic concerns while reading plant cells via circulation cytometric tools, the e-book is going directly to talk about examples of program in plant genetics, genomic research, cellphone cycle research, marine organism research and breeding stories. With its record of common analyzing and a thesaurus of phrases, this primary reference on FCM in vegetation fills a true hole by means of supplying first-hand sensible tricks for the becoming group of plant geneticists.
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4 The Development of Cell Sorting Although impedance (Coulter) counters and optical flow cytometers could analyze hundreds of cells/second, providing a high enough data acquisition rate to be 7 8 1 Cytometry and Cytometers: Development and Growth useful for clinical use, microscope-based static cytometers offered a significant advantage. A system with computer-controlled stage motion could be programmed to reposition a cell on a slide within the field of view of the objective (Stein et al. 1969), allowing the cell to be identified or otherwise characterized by visual observation; it was, initially, not possible to extract cells with known measured characteristics from a flow cytometer.