By Alisher Touraev, Brian P. Forster, Shri Mohan Jain
The discovery of haploid Datura vegetation in 1964 initiated nice pleasure in plant breeding and genetics groups. fresh years have witnessed a resurgence in actions specially in constructing protocols, deciding upon genes and mechanisms and big scale advertisement take up.
The id of controlling genes has pushed practical genomic stories which now hyperlink in with reports in gene expression, metabolism and alterations in mobile ultra-structure. worldwide absorb by means of plant breeders has been no much less outstanding and important haploid applied sciences is more and more patent secure. the serious task in haploid study has additionally ended in unforeseen findings with novel applications.
The middle of this booklet relies at the overseas symposium on "Haploidy in larger vegetation III", (Vienna 2006), which attracted best overseas specialists within the box. different invited contributions were incorporated to supply a rounded view of activities.
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Kluwer, Dordrecht/Boston/London, pp 275–281 Mathys-Rochon E (2005) Secreted molecules and their role in embryo formation in plants: a minireview. Acta Biol Crac Ser Bot 47:23–29 Progress in Doubled Haploid Technology in Higher Plants 27 Matzk F (1991) A novel approach to differentiated embryos in the absence of endosperm. Sex Plant Reprod 4:88–90 Matzk F, Mahn A (1994) Improved techniques for haploid production in wheat using chromosome elimination. Plant Breed 113:125–129 Matzk F, Meyer HM, Bäumlein H, Balzer HJ (1995) A novel approach to the analysis of the initiation of embryo development in Gramineae.
Kluwer, Dordrecht/Boston/London, pp 155–160 Ritala A, Mannonen L, Oksman-Caldentey K-M (2001) Factors affecting the regeneration capacity of isolated barley microspores. Plant Cell Reprod 20:403–407 Röber FK (1999) Fortpflanzungsbiologische und geneische Untersuchungen mit RFLP-Markern zur in-vivo-Haploideninduktion bei Mais. Dissertation, University of Hohenheim. Grauer Verlag, Stuttgart, pp. 124 Röber FK, Gordillo GA, Geiger HH (2005) In vivo haploid induction in maize – Performance of new inducers and significance of doubled haploid line in hybrid breeding.
The chapter will concentrate on critical aspects of doubled haploid induction using unfertilized female gametophytes, while emphasizing the numerous and recent literature. Developmental Stage at Inoculation The pollen developmental stage is one of most important factors in androgenesis. Late uni-cellular microspore is often the most responsive target for re-programming a pathway from gametophytic into sporophytic. There is a general opinion that, in contrast to in vitro androgenesis, it is possible to induce a gynogenic response from ovules over a broad range of developmental stages.