By Michael J. Soares, Joan S. Hunt
Placenta learn has stepped forward speedily during the last numerous many years through benefiting from technical advances, equivalent to microarray research, opposite transcriptase polymerase chain response, protein research, and in situ hybridization. In Placenta and Trophoblast: tools and Protocols, Volumes 1 & 2, across the world well-known investigators describe state of the art laboratory ideas for the research of the trophoblast and placental biology. The thoughts awarded variety from experimental animal types, to animal and human placental organ and mobilephone tradition platforms, to morphological, biochemical, and molecular thoughts for assessing trophoblast/placental progress, differentiation, and serve as. quantity 1 presents quite simply reproducible protocols for learning embryo-uterine implantation, trophoblast mobile improvement, and the association and molecular characterization of the placenta. Highlights comprise thoughts for the isolation and tradition of trophoblast cells from primates, ruminants, and rodents, and distinct suggestions to the molecular and mobile research of the placental phenotype. A significant other moment quantity concentrates on equipment for investigating placental functionality.
finished and state of the art, Placenta and Trophoblast: tools and Protocols, Volumes 1 & 2 offer researchers a company starting place for winning mobile and molecular research of the placenta and the institution of pregnancy.
Read or Download Placenta and Trophoblast: Methods and Protocols Volume 1 PDF
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Extra info for Placenta and Trophoblast: Methods and Protocols Volume 1
Example text
Hummel, K. , and Law, L. W. (1940) The relation of mating, ovulation and estrous smear in the house mouse to time of day. Anat. Rec. 76, 39–54. 34 Deb, Reese, and Paria 3. Carson, D. , Dey, S. , et al. (2000) Embryo implantation. Dev. Biol. 223, 217–237. 4. Dey, S. K. (1996) Implantation. In: Reproductive Endocrinology, Surgery and Technology (Adashi, E. , Rock, J. ), LippincottRaven, New York: pp. 421–434. 5. Yoshinaga, K. and Adams, C. E. (1966) Delayed implantation in spayed, progesterone treated adult mouse.
INDUCTION OF DECIDUALIZATION BY ARTIFICIAL MEANS Artificial decidualization is usually induced by injecting sesame seed (or corn) oil or by placing a silk thread inside the uterus. Injection of sesame seed oil inside the uterus is a less invasive procedure and a preferred method to induce decidualization. 1. Fill a plastic 1-mL syringe attached to a 27-gauge needle with sesame seed oil. 2. ). 3. Hold the uterine horn with a pair of forceps very close to the tip (slightly below the utero–tubal junction) and slowly inject about 20 μL of oil inside the uterine lumen.
Preparation of an Avertin working solution (20 mg/mL): a. 5 mL normal saline in a beaker. b. Seal container with parafilm, wrap in foil to exclude light, and stir on magnetic stirrer for about 12 h or until dissolved. c. Avertin working solutions must be kept refrigerated in a dark bottle until used and should be replaced at least every month. d. 2-μm filter and store at 4°C. Working solutions should be replaced at least every month. 3. 6 mg/g body weight). 75 mL/mouse administered intraperitoneally (see Note 11).