By K. Kitagawa, D. R. Colman (auth.), S. Salvati (eds.)
The ailments that fall below the generalized workforce of demyelinating ailments -Multiple Sclerosis, Leukodystrophyes, Encephalomyelitis-are the point of interest of globally situation. This quantity comprises papers offered by way of prime scientists who attended the NATO complicated study Workshop held on the Istituto Superiore di SanitA, Rome, March 1-4, 1993. This publication is an replace of the former one released in 1987 of the study mentioned at the same assembly held in 1986. It was once made up our minds to carry this second assembly in view that there was nice growth within the advances in figuring out the myelinogenesis technique within the final 5 years. The workshop collected jointly scientists from many fields similar to mobile and molecular biology, immunology, pathology, virology and naturally scientific neurology. Stimulating rules have been exchanged within the wish that extra wisdom of demyelinating illnesses may end up in new theraupetic techniques. even supposing the workshop was once by and large just like the former one, this time there has been extra emphasis on experimental versions and medical points. within the former using animal and mobile versions as instruments for realizing the pathological mechanisms associated with human affliction have been mentioned; within the latter the clinicians defined the filtering down of simple study to medical therapy The booklet of this interdisciplinary alternate is to make recognized the result of the latest examine one of the investigators from allover the area considering those studies.
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Additional resources for A Multidisciplinary Approach to Myelin Diseases II
Technique 2:221-236. , Zoidl, G. , 1992, Coexpression of a new 22kD Schwann cell protein with other peripheral myelin genes during regeneration and development, Abstract of the Proceedings 0/ the 19th GlJttingen Neurobiology Conference, ed. , Georg Thieme Verlag, Stuttgart, New York. , 1993, Coexpression of the PMP22 gene with MBP and Po during de novo myelination and nerve repair, Glia : 8:256-264. , 1990, Characterization of mutations in the factor VllI gene by direct sequencing from amplified genomic DNA, Genomics 6: 65-71.
Histochemical and immunohistochemical analysis of adult brain from these two lines showed oligodendroglial-specific expression of the trans gene. This was confirmed by assaying the 6-galactosidase activity in an highly enriched oligodendrocyte population purified from the MBP-lacZ animals. These data demonstrated that the 256bp proximal region of the MBP gene promoter is sufficient to confere oligodendroglial specific transcription. Although the transgene construct contains, in addition to the 256bp sequence upstream from the MBP major transcription site, the 90 first bp from exon I b, in situ hybridization experiments with a lacZ cRNA probe showed that the transgene RNA was strictly located at the oligodendrocyte cell bodies, suggesting that the 5' region of exon lb of the MBP gene is not responsible for the migration of the MBP transcripts.
Submitted. , 1979, Studies on cultured rat cells. I. Establishment of purified populations from cultures of peripheral nerve, Brain Res. 165:105-118. , 1984, Construction and application of a highly transmissable murine retrovirus shuttle vector, MoL CelL Bioi. 7: 1053-1062. 72:143-151. , 1986, Powerful and versatile enhancer-promoter unit for mammailian expression vectors, Gene 45:101. Natl. Acad. , USA, 87:2725-2729. , 1990, Strategies for obtaining high level expression in mammalian cells. Technique 2:221-236.
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